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I have a trait data set including two parents and one hundred offspring. I want to see whether the trait of the offspring are significant falling out of the trait ranges of the two parents.

So I was thinking to use Dunnett's test to do this comparisons, and then I have several questions:

(1) Can I use the trait values from the two parents (with three replicates each) as one control group? Each offspring with three replicates as one treatment? Can I compare them like this?

(2) Do I need to consider the false discovery rate (FDR) for Dunnett's test? If yes, how should I calculate this?

(3) Can I use the DunnettTests package in R? How can I use it to estimate the FDR?

I provide part of the data here:

   Isolate   Set Replicate  Mean_Radius
   Parent1   Set1   rep1    6.45466
   Parent1   Set1   rep2    6.30849
   Parent1   Set1   rep3    6.03659
   Parent2   Set1   rep1    7.50988
   Parent2   Set1   rep2    7.20987
   Parent2   Set1   rep3    7.45964
Indiv123.1  Set24   rep1    6.704253
Indiv123.1  Set24   rep2    5.890786
Indiv123.1  Set24   rep3    5.826617
Indiv126.1  Set24   rep1    4.89346
Indiv126.1  Set24   rep2    4.857164
Indiv126.1  Set24   rep3    4.993312
Indiv127.1  Set24   rep1    6.394363
Indiv127.1  Set24   rep2    6.671449
Indiv127.1  Set24   rep3    6.426548
Indiv127.2  Set24   rep1    6.519967
Indiv127.2  Set24   rep2    6.896463
Indiv127.2  Set24   rep3    6.837494
Indiv128.1  Set24   rep1    6.428241
Indiv128.1  Set24   rep2    6.23253
Indiv128.1  Set24   rep3    6.548339
Indiv128.2  Set24   rep1    7.343394
Indiv128.2  Set24   rep2    6.993254
Indiv128.2  Set24   rep3    7.221563
Indiv129.2  Set24   rep1    7.052779
Indiv129.2  Set24   rep2    6.868278
Indiv129.2  Set24   rep3    7.130719
Indiv130.1  Set24   rep1    6.340808
Indiv130.1  Set24   rep2    6.607034
Indiv130.1  Set24   rep3    6.813665
Indiv131.1  Set24   rep1    7.696813
Indiv131.1  Set24   rep2    8.306846
Indiv131.1  Set24   rep3    8.106084
Indiv133.1  Set24   rep1    7.578244
Indiv133.1  Set24   rep2    7.698663
Indiv133.1  Set24   rep3    7.638021
Nick Cox
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Zzzz
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  • Hi @Zzzz, would you mind offer the shape of your data? How many parents and offsprings are. And what the traits number, and etc. – Bill Chen Nov 11 '19 at 17:16
  • Hi @BillChen, thank you for replying me! I added a part of the data in the text. I have two parents and 250 offsprings (the Indiv ones in the data) in total. The data is more or less in normal distribution (tested by qqplots, not shown). – Zzzz Nov 11 '19 at 17:38

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