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Questions tagged [imaging]

The process of gathering visual studies of tissue or the body for purposes of research or diagnostics.

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What am I looking at in Franklin's Photo 51 of DNA?

Here's Rosalind Franklin's famous Photo 51, the X-ray diffraction image of DNA from which Watson and Crick deduced its structure: My understanding is that it depicts a short segment of DNA shown from the side (so the axis that the strands wind…
Robert
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Can we determine a person's age by dating methods or other means?

There are many sports restricted to players of a certain age (e.g. 16 and under, 18 and under, etc.) What if a person is older and fakes his birth certificate to say that he is younger? Is there any way to expose this person? Can we determine such a…
Tom
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Can I image Coomassie and GFP in gels at the same time with a fluorescence scanner?

I'm working with a GFP-tagged protein and am routinely using a fluorescence imager (GE Typhoon) and a standard optical scanner to capture fluorescent and absorption images, respectively, of my SDS-PAGE gels. The Typhoon supports multiple channels,…
Nick T
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Can this image be the result of a Western Blot?

I am currently researching image manipulation in the life sciences and stumbled upon a Western Blot looking very similar to the image below. For creating the image I have cut out the blot using the Fuzzy Select Tool of GIMP and then greatly adjusted…
Ralf Thaenert
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Software for counting fly eggs

Is there any software that could be used to rapidly (quicker than humans) count the eggs laid by Drosophila on a substrate by placing them under a microscope with a camera attached? The eggs are laid on the surface of the food and have a fairly…
rg255
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What is the response vector in the fMRI GLM model?

In fMRI, univariate analysis typically makes use of the general linear model (GLM), where the relation between experimental condition and BOLD activity is estimated with a linear regression model for each voxel separately ("mass univariate model").…
Pugl
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Ultrasound during pregnancy

In a hospital I can see a long queue of pregnant women waiting for their turn for ultrasound. Is it safe to go through the ultrasound during pregnancy, especially during last few weeks? Is ultrasound really required? What if it is not done? How…
gpuguy
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Why do water molecules diffuse along axons direction?

I am studying tractography technique which aims to reconstruct bundles of axons in brain by following the diffusion direction of water. It is very interesting because it is non-invasive. It exploits the difference between grey matter and white…
Manuela
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Automatic cropping of collimated digital X-ray images

Do you have any suggestions for a library which offers an algorithm to crop digital X-ray images automatically? We receive full-size images (43x43cm) from a detector, independent of the collimator settings. If the collimator has been closed to…
gehho
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Inhibitory functional connectivity

Functional connectivity may be defined as »the temporal correlation between spatially remote neurophysiological events, expressed as deviation from statistical independence across these events in distributed neuronal groups and areas« or…
Hans-Peter Stricker
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Microscopy Book Suggestions

I've learned programming through great book recommendations; many from the Stack Exchange series of sites. I'm hoping to take this approach in order to gain a fundamental understanding of how fluorescence microscopy and sub-diffraction limit imaging…
ejstronge
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Difference between Diffuse Optical Tomography and Optical Tomography

What are there the differences between these two modalities? Diffuse Optical Tomography (DOT) Optical Tomography (OT) Are there forms of OT that are not DOT?
Amelio Vazquez-Reina
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How to set threshold in ImageJ for quantifying relative fluorescence?

I have taken images from two different samples that have uptaken EdU during DNA synthesis (S-phase). The experiment setup is to find which condition had uptaken more EdU. Therefore I am detecting EdU with fluorescent to be able to quantify them in…
Parham
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Can we see the cell cycle of the cells administered using IVIS-like device?

My question; Can we measure the cell cycle of the administered cells from outside the body, while the recipient is still alive ?　Preferably, by using a device such as IVIS that can read fluorescent labelling in the body non-invasively. In this…
Blue Various
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MRI maximum field strength

More tesla = more resolution, is there limit of human body of how much strong magnetic fields it can handle safely which means there is limit to how strong MRI magnets we can use? Engineering problems aside, could we scan live humans if we had 20 or…
wav scientist
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