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Questions tagged [chromatin]

Chromatin is the complex of DNA, histones, and other scaffolding proteins that make up the chromosomes, which in eukaryotes is located in the nucleus of the cell.

Chromatin is the complex of DNA, histones, and other scaffolding proteins that make up a chromosome, which in Eukaryotes is located in the nucleus of the cell.

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Regulation of chromatin structure

Recently, I reviewed the different levels of chromatin structure. The primary level is nucleosomes, where DNA is bound to histones, and has structural similarity to "beads on a string." The secondary level is a 30nm fiber, and the tertiary level is…
Daniel Standage
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How much is known about dinosaur DNA condensation and crosslinking?

The conclusion section of Bailleul et al. (2020) Evidence of proteins, chromosomes and chemical markers of DNA in exceptionally preserved dinosaur cartilage is: The identification of chemical markers of DNA in Hypacrosaurus suggest it may preserve…
uhoh
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Do nucleosomes ever completely unwrap during transcription?

In eukaryotic transcription will the nucleosomes ever completely unwind the DNA and the histone complex disassemble? If an operon is more 160 base pairs it seems it must.
vajra78
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DNA-DNA cross-linking with formaldehyde?

The 3C (chromosome conformation capture) technology for studying chromatin 3D organization starts by a cross-linking step using formaldehyde to find segments of DNA interacting. In my understanding the cross-linking happens only between the DNA and…
cagliari2005
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What is the mechanism by which lamins regulate gene expression?

The heterochromatin is generally localized at the nuclear periphery (also near nuclear lamina), whereas active genes are preferentially found in the nuclear interior. Children with Hutchinson-Gilford syndrome have a mutation in a LMNA gene, which…
user114141
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What happens after the purification step in Hi-C sequencing?

I am a statistician reading an article on Hi-C, and I am trying to better understand one of the steps in the DNA isolation and sequencing process. Since I'm a statistician, please try to avoid too much jargon in your answers, and if you must use…
Lewkrr
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Bacterial chromatin binding data?

I'm looking for data - maybe CHP^2 data that shows chromatin binding to a prokaryotic genome under some specific conditions. Can anyone point me to a source?
shigeta
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Transcriptionally-mediated DNA damage

I'm researching the genetics of brain cancer, and finding a huge number of mutations in voltage-gated channels. It stands to reason that some of this DNA damage is due to the DNA being transcribed heavily, or in an open chromatin conformation more…
Michael K
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How can chromatin state be measured?

I have some RNA-Seq data and I'd like to align it to the physical genome and see which sections of chromatin are geometrically open and being transcribed. The data are already sequence-aligned, and every Google search I do for tools that might help…
Michael K
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Why is it important to study chromatin to understand cancer?

Many labs and many projects in biology institutes and university departments have been starting to study chromatin. Chromatin states, chromatin interactions, chromatin loopings, chromatin behaviours, etc. seem to have become the new priority of…
DavideChicco.it
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What are "anchor regions" in the human genome?

I've been reading this paper entitled "A high-resolution map of the three-dimensional chromatin interactome in human cells", about interactome in 3D chromatin. We next applied the above algorithm to the 518,032 anchor regions in the human genome,…
DavideChicco.it
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Chromatin shearing: what is it and what are the effects of high-sensitivity?

I am reading a paper which discusses a complex (MSL-DCC) involved in dosage compensation of the drosophila X-chromosome. Descriptions of the complex's structure and function are given in the papers introduction. To start with, could someone…
rg255
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In chromatin, what are the differences among states, interactions, and structures?

I am new to the study of chromatin, and I am trying to understand what people mean when they write about chromatin states, chromatin interactions, and chromatin structures. What are the difference among these? Is there someone available to explain…
DavideChicco.it
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Can untargeted metabolomics detect epigenomic changes such as methylation?

My main focus is transcriptomics analysis and very recently I have also started working on metabolomics. From my transcriptomic data it looks like there is a gene/protein that could potentially generate epigenetic modifications. I also have…
Sari
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Why does cell waste energy in meiosis, between meiosis 1&2

As far as I have learnt about meiosis I have read that anaphase 1 is followed by telophase 2 where chromosomes change back to reticulum.but in the very next stage i.e prophase 2 they again start condensing to form chromosomes again.i have also heard…
Adithya Eshwarla
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